Figure 4.

Detection of antioxidant enzymes is increased in EAE-induced lesions. (A, C, E, G, I) Representative confocal images of NAWM or EAE-induced mice labeled with CD68 for microglia/macrophage (green), antioxidant enzyme of interest (red), OLIG2 for oligodendrocyte lineage cells (white). Dotted line indicates the lesion ROI selected for image analysis. (B, D, F) Bar graphs comparing the percent of ROI that is CD68⁺, antioxidant enzyme of interest, and CD68⁺ antioxidant enzyme⁺. (H) Bar graph comparing the number of GPX4⁺ cells per mm² × 10² of ROI and percent of ROI that is CD68⁺ GPX4⁺ (J) Bar graph representing number of OLIG2 + cells per mm² × 10² of ROI and percent of ROI that is OLIG2⁺ GPX4⁺. Scale bar = 100 µm. Data are shown as mean ± S.D, n = 4 mice. Significance indicated as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, two-tailed, paired student’s t-test.