TABLE 3.

Catabolism of three inhibitors, acrylic acid, 2-bromooctanoic acid, and 4-pentenoic acid, after 48 h of second-step cultivation at 30°C

Inhibitor	Carbon sourcea	Initial concn (mM ± SD) of inhibitor in medium	Concn (mM ± SD) of inhibitor remaining in medium after 48 h	% Inhibitor catabolized after 48 h
Acrylic acid	Fructose	1.93	NDb	100
		3.95	ND	100
	Octanoate	2.07	ND	100
		3.67	ND	100
		5.22	ND	100
2-Bromooctanoic acid	Fructose	0.237 ± 0.009	0.168 ± 0.015	29
		1.311 ± 0.021	1.214 ± 0.056	8
		2.497 ± 0.022	2.409 ± 0.031	4
	Octanoate	2.521 ± 0.040	2.471 ± 0.038	2
		5.368 ± 0.274	5.328 ± 0.285	1
4-Pentenoic acid	Fructose	3.42	ND	100
		4.96	ND	100
	Octanoate	3.62	ND	100
		4.99	ND	100

^aThe fed concentration was 70 mM for all experiments with fructose, and the fed concentration was 40 mM for all experiments with octanoate. All media initially contained 1 g of ammonium sulfate/liter. 

^bND, not detected.