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. 2022 Jul 13;13:932388. doi: 10.3389/fimmu.2022.932388

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Copyright © 2022 Dahlgren, Plumb, Niss, Lahl, Brunak and Johansson-Lindbom

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tfh- and Th1-cell development is supported by IFNAR-signaling in hematopoietic cells distinct from cognate CD4 T cells. Equal numbers (250 000) of CTV-labelled WT and Ifnar1^-/- OT-II cells were co-transferred into Ifnar1^-/- BM chimeric recipient mice and spleens were analyzed 3 days after immunization with OVA/poly I:C. (A) Representative CTV dot plots showing gating strategy of OT-II cells from mice receiving either WT or Ifnar1^-/- BM. (B) Representative CTV profiles of OT-II cells from all experimental groups. Arrows indicate median division in the full WT group. (C) Percentages (mean ± SD) of OT-II cells in indicated cell cycle number as determined by CTV-dilution. Verticle lines indicate median division in the full WT group. (D–G) Percentage of T-bet⁺ (D), IFN-γ⁺ (E), Bcl6⁺ (F), and CXCR5⁺ (G) WT and Ifnar1^-/- OT-II cells, respectively. Results are pooled from two individual experiments consisting of a total of 6-7 mice per group. Each pair of symbols represents one mouse (D–G).