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. 2022 Jul 13;10:899368. doi: 10.3389/fcell.2022.899368

FIGURE 3.

FIGURE 3

iPSC-derived lung organoid differentiation: (A)—The protocol starts with the induction of iPSC colonies to form the definitive endoderm, which is then differentiated into foregut spheroids. These spheroids are then embedded in a gel basal matrix and further differentiated into LOs. (B)—Bright-field images of the different stages of LO development from iPSCs.