TABLE 1.

IC₅₀ and K_i values for inhibition of PDE3, PDE5, ABCC4, and ABCC5 at temperatures ranging from 37°C to 20°C.

Milrinone
	PDE3		PDE5		ABCC4		ABCC5
Temperature (°C)	IC50	Ki	IC50	Ki	IC50	Ki	IC50	Ki
37	2.99 ± 1.28	0.14 ± 0.059	336 ± 58.60‡	85.3 ± 14.87‡	58.9 ± 13.09‡●	35.69 ± 7.94 ‡●	22.8 ± 1.52	12.91 ± 0.86
34	1.77 ± 0.72 ‡	0.081 ± 0.033‡	255 ± 29.84‡	64.6 ± 7.57‡	67.0 ± 22.65‡	40.65 ± 13.73‡	17,8E ± 1.73‡	10.04 ± 0.96‡
32	1.30 ± 0.36‡	0.060 ± 0.016‡	280 ± 48.33‡	70.9 ± 12.26‡	32.9 ± 14.61	19.95 ± 8.86	17.2 ± 3.02‡	9.70 ± 1.71‡
28	0.82 ± 0.31	0.038 ± 0.014	305 ± 72.47‡	77.4 ± 18.39‡	37.4 ± 24.52	22.67 ± 14.86	18.2 ± 2.03‡●	10.26 ± 1.15‡●
24	1.91 ± 0.73	0.087 ± 0.034	253 ± 32.92‡●	64.2 ± 8.35‡●	-		8.62 ± 2.69#	4.87 ± 1.52#
20	2.15 ± 0.65	0.099 ± 0.030	330 ± 58.11‡●	83.8 ± 14.75‡●	-		7.35 ± 3.51#	4.16 ± 1.98#

^#Significant difference (p-value < 0.05) when compared to normothermic control.

^‡Significant difference (p < 0.05) when compared to amrinone.

^●Significant difference (p < 0.05) when compared to levosimendan.

Values are presented as means ± SEM and given in µM.