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Physical map of the flanking regions of rtxA. (A) Seven independent overlapping pLAFR1 cosmids containing rtxA from a genomic library of B. elkanii USDA94. (B) In the 28,401 bp of sequence obtained, an fixGHIS cluster, noeE, nodPQ, rtxA, and six ORFs were found. Restriction sites: A, ApaI; B, BamHI; E, EcoRI; H, HindIII; P, PstI. C, probe used for colony and Southern hybridization.
