TABLE 4.
Case number | Sample | Cellularitya (1–5) |
Class of necrosisb (0–5) |
Number of hyperplastic vessels counted (n) | Habitats involved (ordered by prevalence inside VOI)c |
2 | A | 1 | 0 | 0 | 7, 8 |
B | 4 | 0 | 43 | 2 | |
C | 4 | 1 | 21 | 5 | |
5 | A | 3 | 2 | 1 | 6 |
B | 4 | 4 | 1 | 2, 5 | |
C | 3 | 5 | 3 | 5, 7, 8 | |
D§ | 3 | 3 | 6 | 5, 6 | |
8 | A | 2 | 0 | 0 | 1 |
B | 2 | 0 | 0 | 1 | |
C | 2 | 0 | 1 | 1 | |
D | 2 | 0 | 0 | 1 | |
9 | A | 5 | 2 | 20 | 1 |
B | 5 | 2 | 19 | 1 | |
10 | A | 1 | 0 | 0 | 7, 8 |
B | 3 | 1 | 8 | 2 | |
12 | A | 1 | 0 | 4 | 8, 2, 6 |
B | 3 | 2 | 4 | 2, 6 | |
C | 4 | 1 | 8 | 2 | |
13 | A | 1 | 0 | 0 | 7 |
B | 1 | 0 | 0 | 5 | |
C | 3 | 0 | 0 | 3 | |
D | 3 | 0 | 2 | 1,3 | |
14 | A | 3 | 0 | 5 | 7,8,6 |
B | 4 | 0 | 11 | 6 | |
16 | A | 1 | 0 | 0 | 7, 8 |
B | 3 | 0 | 28 | 1 | |
C | 5 | 1 | 11 | 2 | |
D | N/A* | 5 | N/A* | 5, 7 | |
17 | A | 4 | 0 | 6 | 2 |
B | 4 | 1 | 7 | 2 | |
C | 4 | 0 | 4 | 6, 2 |
Features assessed in the present analysis were: cellularity, extent of necrosis, and number of hyperplastic vessels counted.
To address the issue of length variability among different bioptic specimens and to maximize the correspondence with the target point set on the stereotactic planning system, only the central portion (extending 5 mm along the greatest axis of the specimen in its largest section available) was analyzed in each sample.
The corresponding spatial tumor imaging habitats involved are reported in the last column, ordered accordingly to their relative prevalence inside of the 5 mm diameter volume of interest.
N/A, not applicable.
aCellularity was graded with a score from 1 (seemingly normal representation) to 5 (extremely high cellular density).
bClasses considered for necrosis (expressed as a rate over the whole area considered for each specimen) were the following: 0 (0%), 1 (≤10%), 2 (11–25%), 3 (26–50%), 4 (51–75%), 5 (76–100%).
cA 5 mm diameter volume of interest (VOI) was drawn according to the exact stereotactic coordinates of each target and needle window orientation, then carefully verified also in the coregistered in the early post-operative CT scan. In this column, the included habitats are reported. Since the spatial habitat maps were not available at the time of planning, final sampling VOI ended at the intersection of multiple clusters in many cases; in the latter case, clusters are listed in order of maximum volumetric representation inside of the VOI.
§This sample was only 4 mm long.
*It was not possible to calculate these features since the sample was completely represented by necrosis.