FIG. 6.

(a) Native gel of particulate preparations from P. luminescens K122 and K122 expressing recombinant Tca (pA1) and Tcd (pD1). Note that preparations from the acceptor strain P. luminescens K122 migrate as two clearly separable complexes, whereas coexpression of W14 Tca or Tcd results in a single native complex being produced by recombinant P. luminescens K122. The asterisk represents the recombinant Tca complex excised from the gel and reanalyzed by SDS-PAGE. (b) Denaturing gel electrophoresis (SDS-PAGE) of the same K122 and K122-Tca preparations as those analyzed in panel a. Note that the native Tca complex excised from the gel in panel a contains three predominant polypeptides (arrowheads). Note also that this native complex forms visible particles under TEM examination (see Fig. 8d).