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Gating strategy to identify peripheral blood monocyte subsets. (A) Monocytes were gated using forward- and side-scatter properties. The 3 subsets were characterized through expression of CD14 and CD16 markers (a: classical CD14++CD16–, b: intermediate CD14++CD16+, and c: non-classical CD14+CD16++). (B) LILRB1 or LILRB2 expression levels on the 3 monocyte subsets were defined using histogram geometric mean.
