FIG. 1.

Sequence of the cry1Da1 upstream region, including the overlapping promoters and the initiation of translation. The upstream oligonucleotide used to amplify this region was complementary to the T7 primer region and extended into the multiple cloning site of pUC18 which was present in the cloned cry1Da1 fragment used for PCR (see Materials and Methods). The downstream oligonucleotide extended from within the cry1Da1 coding region through the ribosome binding site. The ribosome binding sequence is overlined and was changed to AAGGAGGT by modifying the oligonucleotide primer as described in Materials and Methods. The positions of the overlapping promoter regions (−10 and −35) for the ς^E and ς^K forms of RNA polymerase are indicated. The GenBank accession number of the sequence is AF337948.