FIG. 2.

(A) Autoradiograms of Northern gels of RNAs from sporulating cells sampled after the addition of rifampin at the times indicated (see Materials and Methods). Hybridization was to a ³²P-labeled cry1Da1 (Cry1D) or ³²P-labeled cry1Ab3 (Cry1Ab) probe. (B) X-ray film was quantified with a phosphorimager, and the percentages of the initial values were plotted on a log scale versus time in order to calculate mRNA half-lives of cry1Ab3 mRNA (○) and cry1Da1 mRNA (□). Standard errors are indicated by error bars.