FIG. 4.

Immunoblots of trypsin-treated extracts from B. thuringiensis subsp. aizawai HD133 grown in G-tris medium (G) (25 μg of protein) or LB medium (LB) (30 μg of protein) until 30% of the cells in G-tris medium or ca. 25% of the cells in LB medium contained phase-gray endospores. Samples were prepared as described in Materials and Methods. Lanes 1 and 2 were treated with the Cry1D antibody, lanes 3 and 4 were treated with the Cry1Ca1 antibody, and lanes 5 and 6 were treated with the Cry1Ab3 antibody. The blots were scanned and quantitated as described in Materials and Methods.