FIG. 5.

Immunoblots of trypsin-treated extracts from purified inclusions prepared from B. thuringiensis subsp. aizawai HD133 grown and sporulated in G-tris medium (G) or LB medium (LB). Preparations were treated with the Cry1Ab3, Cry1Ca1, and Cry1Da1 antibodies as described in Materials and Methods. Lanes 1 to 4 were treated with Cry1Da1 antibody, lanes 5 to 8 were treated with Cry1Ca1 antibody, and lanes 9 and 10 were treated with Cry1Ab3 antibody. Lanes 1, 2, 5, 6, and 9 contained inclusions from G-tris medium; lanes 1 and 2 were duplicates, as were lanes 5 and 6. Lanes 3, 4, 7, 8, and 10 contained inclusions from LB medium; lanes 3 and 4 were duplicates, as were lanes 7 and 8.