Figure 3.

Discovery of novel cell surface proteins with antibodies. (A) Selection of mAbs directly binding to cancer cells by phage display and that do not bind to untransformed cells. Next, their target is identified by mass spectrometry analysis. (B) Determination of the surfaceome of cancer cells expressing an oncogene (e.g., mutant KRAS, KRAS^MUT) compared to the same cell line not expressing the oncogene (e.g., wild-type KRAS, KRAS^WT) to discover oncogene-dependent cell surface proteins by mass spectrometry analysis. mAbs are then selected by phage display against these newly discovered proteins.