Table 5.
The accuracy and intra-day, inter-day precision of wogonosides, apigenin-7-O-glucuronide, and baicalin in the bile and blood matrix.
| Analytes | QCa | Intra-day | Inter-day | ||||||
|---|---|---|---|---|---|---|---|---|---|
|
| |||||||||
| Accuracy (%) | Precision (RSD %) | Accuracy (%) | Precision (RSD %) | ||||||
|
| |||||||||
| Bile | Blood | Bile | Blood | Bile | Blood | Bile | Blood | ||
| Wogonoside | Low | 108.4 | 99.9 | 2.0 | 11.2 | 105.9 | 94.4 | 4.4 | 6.1 |
| Medium | 102.5 | 99.1 | 4.3 | 5.6 | 104.1 | 96.3 | 2.7 | 7.7 | |
| High | 99 | 96.7 | 8.3 | 3.1 | 99.7 | 94.1 | 4.0 | 4.4 | |
| Apigenin-7-O-glucuronide | Low | 107.5 | 111.1 | 1.7 | 2.0 | 111.4 | 94.5 | 3.2 | 5.2 |
| Medium | 113.5 | 98.7 | 6.9 | 12.0 | 108.0 | 96.4 | 4.9 | 11.0 | |
| High | 88.1 | 94.8 | 4.7 | 9.2 | 95.2 | 93.0 | 3.0 | 10.6 | |
| Baicalin | Low | 105.9 | 99.5 | 4.7 | 9.9 | 104.2 | 92.9 | 3.2 | 7.7 |
| Medium | 98.0 | 94.2 | 4.8 | 6.7 | 102.0 | 88.5 | 3.7 | 10.6 | |
| High | 95.3 | 97.1 | 7.9 | 6.9 | 94.0 | 94.4 | 4.8 | 8.3 | |
a
Low, medium, and high were 10.0, 156.0, 2500.0 nM in the bile and 1.56, 25.0, and 2000.0 nM in the blood, respectively.