Table 3.
Bacterial Strains and plasmids
| Plasmid or Strain | Relevant genotype or features | Source, construction or reference | |
|---|---|---|---|
| Strains | |||
| E. coli | |||
| HB101 | F− mcrB mrr hsdS20(rB− mB−) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20 | B. Dupuy | |
| C. difficile | |||
| 630Δerm | ErmS derivative of strain 630 | N. Minton [75] | |
| MC310 | 630Δerm spo0A::erm |
[31] | |
| MC324 | 630Δerm pMC123 | [31] | |
| MC848 | 630Δerm spo0A::erm pMC566 | This study | |
| MC849 | 630Δerm spo0A::erm pMC567 | This study | |
| MC855 | 630Δerm spo0A::erm pMC123 | This study | |
| MC961 | 630Δerm spo0A::erm pMC656 | This study | |
| MC962 | 630Δerm spo0A::erm pMC657 | This study | |
| MC981 | 630Δerm spo0A::erm pMC663 | This study | |
| MC1003 | 630Δerm spo0A::erm pMC674 | This study | |
| MC1033 | 630Δerm spo0A::erm pMC684 | This study | |
| MC1036 | 630Δerm spo0A::erm pMC685 | This study | |
| MC1057 | 630Δerm spo0A::erm pMC697 | This study | |
| MC1058 | 630Δerm spo0A::erm pMC698 | This study | |
| MC1059 | 630Δerm spo0A::erm pMC699 | This study | |
| MC1060 | 630Δerm spo0A::erm pMC700 | This study | |
| MC1061 | 630Δerm spo0A::erm pMC701 | This study | |
| MC1062 | 630Δerm spo0A::erm pMC702 | This study | |
| MC1063 | 630Δerm spo0A::erm pMC703 | This study | |
| MC1064 | 630Δerm spo0A::erm pMC704 | This study | |
| MC1184 | 630Δerm spo0A::erm pMC768 | This study | |
| MC1185 | 630Δerm spo0A::erm pMC770 | This study | |
| MC1527 | 630Δerm pMC917 | This study | |
| MC1529 | 630Δerm pMC930 | This study | |
| MC1618 | 630Δerm spo0A::erm pMC732 | This study | |
| MC1619 | 630Δerm spo0A::erm pMC742 | This study | |
| MC1620 | 630Δerm spo0A::erm pMC769 | This study | |
| MC1621 | 630Δerm spo0A::erm pMC771 | This study | |
| MC1664 | 630Δerm spo0A::erm pMC967 | This study | |
| MC1665 | 630Δerm spo0A::erm pMC969 | This study | |
| MC1666 | 630Δerm spo0A::erm pMC970 | This study | |
| MC1670 | 630Δerm spo0A::erm pMC965 | This study | |
| MC1671 | 630Δerm spo0A::erm pMC966 | This study | |
| MC1690 | 630Δerm spo0A::erm pMC971 | This study | |
| MC1711 | 630Δerm spo0A::erm pMC975 | This study | |
| MC1712 | 630Δerm spo0A::erm pMC976 | This study | |
| MC1713 | 630Δerm spo0A::erm pMC986 | This study | |
| MC1778 | 630Δerm spo0A::erm pMC968 | This study | |
| MC1846 | 630Δerm spo0A::erm pMC1055 | This study | |
| MC1904 | 630Δerm spo0A::erm pMC922 | This study | |
| MC1905 | 630Δerm spo0A::erm pMC924 | This study | |
| MC1906 | 630Δerm spo0A::erm pMC944 | This study | |
| MC1991 | 630Δerm spo0A::erm pMC1097 | This study | |
| MC1992 | 630Δerm spo0A::erm pMC1098 | This study | |
| MC1993 | 630Δerm spo0A::erm pMC1099 | This study | |
| MC1994 | 630Δerm spo0A::erm pMC1100 | This study | |
| MC1995 | 630Δerm spo0A::erm pMC1101 | This study | |
| MC1996 | 630Δerm spo0A::erm pMC1102 | This study | |
| MC1997 | 630Δerm spo0A::erm pMC1103 | This study | |
| MC1998 | 630Δerm spo0A::erm pMC1104 | This study | |
| MC1999 | 630Δerm spo0A::erm pMC1105 | This study | |
| MC2000 | 630Δerm spo0A::erm pMC1106 | This study | |
| MC2001 | 630Δerm spo0A::erm pMC1107 | This study | |
| MC2002 | 630Δerm spo0A::erm pMC1108 | This study | |
| MC2003 | 630Δerm spo0A::erm pMC1109 | This study | |
| MC2004 | 630Δerm spo0A::erm pMC1110 | This study | |
| MC2005 | 630Δerm spo0A::erm pMC1111 | This study | |
| Plasmids | |||
| pRK24 | Tra+, Mob+; bla, tet | [76] | |
| pUC19 | Cloning vector; bla | [77] | |
| pMC123 | E. coli-C. difficile shuttle vector; bla, catP | [59] | |
| pMC566 | pMC123 WT Spo0A | This study | |
| pMC567 | pMC123 Spo0A D56A | This study | |
| pMC656 | pMC123 Spo0A N12A | This study | |
| pMC657 | pMC123 Spo0A K13A | This study | |
| pMC663 | pMC123 Spo0A I58A | This study | |
| pMC674 | pMC123 Spo0A 3xFLAG | This study | |
| pMC684 | pMC123 Spo0A V18A | This study | |
| pMC685 | pMC123 Spo0A H61A | This study | |
| pMC697 | pMC123 Spo0A C16A | This study | |
| pMC698 | pMC123 Spo0A E21A | This study | |
| pMC699 | pMC123 Spo0A A35S | This study | |
| pMC700 | pMC123 Spo0A P60A | This study | |
| pMC701 | pMC123 Spo0A A87S | This study | |
| pMC702 | pMC123 Spo0A V88A | This study | |
| pMC703 | pMC123 Spo0A G89A | This study | |
| pMC704 | pMC123 Spo0A K108A | This study | |
| pMC742 | pMC123 Spo0A D91A | This study | |
| pMC768 | pMC123 Spo0A M59A | This study | |
| pMC769 | pMC123 Spo0A L62A | This study | |
| pMC770 | pMC123 Spo0A K92A | This study | |
| pMC771 | pMC123 Spo0A P109A | This study | |
| pMC915 | pAF 256 HupA-SmBit-LgBit | Wiep Klaas Smits [56] | |
| pMC916 | pAF257 SmBit-HupA-LgBit | Wiep Klaas Smits [56] | |
| pMC917 | pAF 259 BitLuc | Wiep Klaas Smits [56] | |
| pMC918 | pAP118 HupA-SmBit-HupA-LgBit | Wiep Klaas Smits [56] | |
| pMC922 | pAP118 Spo0A-SmBit-LgBit | This study | |
| pMC924 | pAP118 SmBit-Spo0A-LgBit | This study | |
| pMC930 | pAF256 SmBit-LgBit | This study | |
| pMC932 | pAF257 Spo0A-SmBit-LgBit | This study | |
| pMC944 | pMC932 Spo0A-SmBit-Spo0A-LgBit | This study | |
| pMC965 | pMC123 Spo0A D11A | This study | |
| pMC966 | pMC123 Spo0A D14A | This study | |
| pMC967 | pMC123 Spo0A F15A | This study | |
| pMC968 | pMC123 Spo0A Q17A | This study | |
| pMC969 | pMC123 Spo0A L19A | This study | |
| pMC970 | pMC123 Spo0A D111A | This study | |
| pMC971 | pMC123 Spo0A D56A 3xFLAG | This study | |
| pMC975 | pMC123 Spo0A K36A | This study | |
| pMC976 | pMC123 Spo0A Q90A | This study | |
| pMC986 | pMC123 Spo0A F110A | This study | |
| pMC1055 | pMC123 Spo0A S86A | This study | |
| pMC1088 | pMC123 Spo0A D10A | This study | |
| pMC1097 | pAF256 Spo0A D10A-SmBit | This study | |
| pMC1098 | pAF256 Spo0A D11A-SmBit | This study | |
| pMC1099 | pAF256 Spo0A D56A-SmBit | This study | |
| pMC1100 | pAF256 Spo0A I58A-SmBit | This study | |
| pMC1101 | pAF256 Spo0A K108A-SmBit | This study | |
| pMC1102 | pAF257 Spo0A D10A-LgBit | This study | |
| pMC1103 | pAF257 Spo0A D11A-LgBit | This study | |
| pMC1104 | pAF257 Spo0A D56A-LgBit | This study | |
| pMC1105 | pAF257 Spo0A I58A-LgBit | This study | |
| pMC1106 | pAF257 Spo0A K108A-LgBit | This study | |
| pMC1107 | pAP118 D10A-SmBit-D10A-LgBit | This study | |
| pMC1108 | pAP118 D11A-SmBit-D11A-LgBit | This study | |
| pMC1109 | pAP118 D56A-SmBit-D56A-LgBit | This study | |
| pMC1110 | pAP118 I58A-SmBit-I58A-LgBit | This study | |
| pMC1111 | pAP118 K108A-SmBit-K108A-LgBit | This study | |