FIG. 3.

Gliotoxin production in Psy1 disruptants of G20-4VIB. Filtrates from malt broth cultures grown for 2 days were extracted with chloroform and subjected to TLC on a plate with a fluorescent background (37). Dark bands indicate UV-absorbing compounds. Lane Std contained a gliotoxin standard. Culture filtrates of wild-type strain G20-4VIB (lane WT) and Psy1disruptants (T strains) derived from this strain were also included. The arrows indicate the positions of gliotoxin (G) and viridin (V). Viridin is a sterol metabolite that is not related to gliotoxin. Band MeG is dimethyl gliotoxin, an inactivated derivative of gliotoxin formed in culture.