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. 2022 Jul 27;10:110. doi: 10.1186/s40168-022-01304-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Microbial community profile of coastal seawater samples at order (a) and genus (b) levels. Bacterial diversity of the natural (T0) and labelled (heavy; H) and unlabelled (light; L) fractions of ¹³C-DMSP seawater incubations was analysed by 16S rRNA gene amplicon (16S) and metagenomics (MG) sequencing. “_1”, “_2” and “_3” after the sample name represent biological replicates. Biological replicates from ¹³C-heavy and ¹³C-light fractions were respectively combined before MG sequencing due to their highly similar 16S rRNA gene community profile shown by DGGE (Fig. S7). Only classes and genera with RA >0.5% in at least one of the conditions are represented. Statistically enriched genera in the incubations with ¹³C-labelled DMSP (13C_T) compared the natural (T0) samples based on 16S data are labelled with an asterisk. Classes and genera with RA <0.5% are grouped into “others”. For 16S and MG data of samples incubated with ¹²C-DMSP (controls), see Fig. S8