Table 1.

Characteristics of DMSP-degrading bacterial strains isolated from seawater samples incubated with DMSP

Strain	Top-hit taxon identified by 16S rRNA gene sequences	Reference straina	Accession number of genomes from sequenced isolates	Homologues in reference strain or genome from sequenced isolateb	DMS production ratec	MeSH productiond	Growth on DMSPe	Class
GY12	Litoreibacter albidus	DSM 26922		dddA, dddC, prpE, acuI, acuK, dmdA, dmdB, dmdC	ND	Y	N	Alphaproteobacteria
MB12-2	Neptunicoccus sediminis	CY02		dddP, prpE, acuI, acuK, dmdA, dmdB, dmdC	6 ± 2	ND	N	Alphaproteobacteria
GY7	Pseudophaeobacter arcticus	DSM 23566		dddW, dddA, dddC, prpE, acuI, acuN, acuK, dmdA, dmdB, dmdC, dmdD	558 ± 260	ND	N	Alphaproteobacteria
MB12-4	Sulfitobacter pontiacus	DSM 10014		dddL, dmdB, dmdC, dmdD	648 ± 153	ND	N	Alphaproteobacteria
GY16	Sulfitobacter pseudonitzschiae	H3		dddL, dddA, dddC, prpE, acuI, acuK, dmdB, dmdC, dmdD	201 ± 40	ND	N	Alphaproteobacteria
D12-10	Alteromonas stellipolaris	LMG 21861		dmdC	60 ± 6	ND	Y	Gammaproteobacteria
GY8	Marinobacter sediminum		JAGTWY000000000	dddL, dddA, dddC, prpE, dmdB, dmdC	4560 ± 785	ND	Y	Gammaproteobacteria
MC12-9	Marinobacter similis	A3d10		dddL, dddA, prpE, acuN, acuK, dmdC	86 ± 3	ND	Y	Gammaproteobacteria
GY20	Pseudoalteromonas hodoensis	H7			ND	Y	N	Gammaproteobacteria
D13-2	Cobetia amphilecti	KMM 296		dddD, dddA, dddC, dddT, prpE, acuI, acuK, dmdC, tmm	1342 ± 101	ND	N	Gammaproteobacteria
MC13-5	Cobetia litoralis		CP073342	dddD, dddA, dddC, dddT, prpE, acuI, dmdC, tmm	1316 ± 94	ND	Y	Gammaproteobacteria
GY6	Amphritea atlantica		CP073344, CP073345	dddD, prpE (2), acuI, acuK, dddB, dddC, dddT, dmdB (4), dmdC, tmm	1173 ± 208	ND	Y	Gammaproteobacteria
D13-1	Marinobacterium rhizophilum		CP073347	dddD, dddP, prpE (2), acuI, acuN, acuK, dddA, dddB, dddC, dddT, dmdB (2), dmdC	1039 ± 21	ND	Y	Gammaproteobacteria
MC13-7	Marinobacterium profundum	PAMC 27536		dddD, dddP, dddA, dddB, dddC, dddT, prpE (2), acuI, dmdB, dmdC	1132 ± 31	ND	Y	Gammaproteobacteria
GY1	Marinomonas atlantica	Cmf 18.22		dddD, dddB, dddC, prpE, acuI, dmdC	59 ± 30	ND	Y	Gammaproteobacteria
MB12-3	Marinomonas foliarum	CECT 7731		dddC, acuI, dmdC	11 ± 0.4	ND	N	Gammaproteobacteria
MB12-11	Marinomonas rhizomae		CP073343	dddD, dddB, dddC, dddT, acuI, dmdC	500 ± 36	ND	Y	Gammaproteobacteria
D13-4	Pseudomonas benzenivorans		CP073346	dddD, dddP, acuK, dddB, dddC, dddT, dmdB, dmdC	2462 ± 123	ND	Y	Gammaproteobacteria
GY22	Pseudomonas leptonychotis	CCM 8849		dddD, dddB, dddC, dddT, dmdB, dmdC	2350 ± 343	ND	Y	Gammaproteobacteria
GY17	Pseudomonas taeanensis	MS-3		dddD, dddP, dddB, dddC, dddT, acuI, dmdB, dmdC, tmm	829 ± 186	ND	Y	Gammaproteobacteria
GY15	Vibrio splendidus	10N.286.45		prpE, acuI, DMSOR	ND	Y	Y	Gammaproteobacteria

^aReference strain: most closely related strain with publicly available genome

^bNumber of prpE and dmdB genes in genomes with multiple copies are indicated in brackets

^cRate of DMSP-dependent DMS production expressed in nmol DMS mg protein⁻¹ h⁻¹

^dY, detectable MeSH production from DMSP. ND, not detected

^eY, growth on DMSP as sole carbon source (Student’s t-test, P < 0.05); N, no growth on DMSP as sole carbon source (P > 0.05)