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. 2022 Jul 26;13:359. doi: 10.1186/s13287-022-03018-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Downregulation of miR-152-5p regulates osteogenic differentiation via promoting autophagy in OVX MMSCs-M. a RUNX2, ALP, OCN were detected by Western blots in groups of NC, inhibitor, inhibitor + 3-MA, inhibitor + siATG5 (n = 3). b QRT-PCR was performed to assess the expression of Runx2, Alp, Ocn in above groups (n = 3). c, d. Alizarin Red S staining and ALP staining and activity of these groups. Data were shown as mean ± SD (p < 0.05*, p < 0.01**, p < 0.0005***, p < 0.0001****)