Fig. 5.

Atg14 is a direct target of miR-152-5p. a The supposed target sequence for miR-152-5p on the 3’-UTR of Atg14. b, c miR-152-5p was overexpressed in sham MMSCs-M by transfecting miR-152-5p mimics, while miR-152-5p inhibitor was used to downregulate the expression of miR-152-5p in OVX MMSCs-M. Western blotting assessed the expression of ATG14 in these groups. d Luciferase activity was performed in MMSCs-M. e Western blotting assays were performed to the expression of protein LC3B and p62 in the groups of inhibitor NC + siNC, miR-152-5p inhibitor + siNC, inhibitor NC + siATG14, miR-152-5p inhibitor + siATG14 (n = 3). f The number of autophagic vacuoles was observed by TEM in the above groups, scar bar = 2um. g QRT-PCR was performed to assess the osteogenic markers in the above groups. Data were shown as mean ± SD (p < 0.05*, p < 0.01**, p < 0.0005***, p < 0.0001****)