Fig. 4.

Effect of different H₂O₂ concentrations on the Keap1-Nrf2 signalling pathway in NPMSCs. a and b Western blotting analysis of Keap1 and Nrf2 expression in NPMSCs exposed to different concentrations of H₂O₂ (n = 3, *P < 0.05, **P < 0.01, one-way ANOVA followed by Bonferroni’s post hoc test. ^#P < 0.05 compared to the 300 μM H₂O₂ group). c and d Western blotting analysis of Brd4 expression in NPMSCs exposed to different concentrations of H₂O₂ (n = 3, *P < 0.05, **P < 0.01, one-way ANOVA followed by Bonferroni’s post hoc test. ^#P < 0.01 compared to the 300 μM H₂O₂ group). e and f Western blotting analysis estimating the effect of 75 μM H₂O₂ pretreatment on Keap1, Nrf2, and Brd4 expression and quantification when NPMSCs were subsequently exposed to 300 μM H₂O₂ (n = 3, *P < 0.05, **P < 0.01, one-way ANOVA followed by Bonferroni’s post hoc test. ^##P < 0.01 compared to the 300 μM H₂O₂ group)