Fig. 4.

C/EBPα regulates the transcriptional activity of the PPARγ promoter. a Bioinformatic prediction results of potential binding sites of C/EBPα in the PPARγ promoter region. b, c ChIP analysis of PPARγ promoter binding sites in BMSCs with OE-Vector and OE-C/EBPα and agarose gel electrophoresis of ChIP–qPCR products. d The potential binding sites of C/EBPα in the promoter of the PPARγ gene and the structural diagram of the luciferase reporter of WT-PPARγ or MUT-PPARγ binding sites. e Analysis of relative luciferase activity. f, g ChIP assay results of C/EBPα binding at the PPARγ promoter region in femoral head tissues of SD rats in the normal and model groups. The ChIP–qPCR products were subjected to agarose gel electrophoresis. **P < 0.01, *P < 0.05, ns = not significant