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. Author manuscript; available in PMC: 2023 Mar 3.
Published in final edited form as: Mol Cell. 2022 Feb 18;82(5):986–1002.e9. doi: 10.1016/j.molcel.2022.01.024

Figure 2. Nanog, Pou5f3, and Sox19b are required for histone acetylation across core histones and for recruitment of p300 and Brd4.

Figure 2.

(A) Heatmaps showing histone acetylation levels across histones at N/P/S-bound active enhancers and promoters of differentially affected zygotic genes. Heatmaps are centered at TSSs (promoters) or Omni-ATAC peak summits (active enhancers) and ranked by Pol II binding intensity within 1kb of the TSS of the associated zygotic genes in wild-type (WT) embryos. (B) Heatmap showing the correlation between changes in transcription and changes in histone acetylation marks in MZnps/WT for N/P/S-bound active enhancers and promoters of zygotic genes. H2A.ZK4/7ac-H2A.Z ratio represents the ratio between H2A.ZK4/7ac and H2A.Z. (C) Heatmaps showing p300 and Brd4 levels at N/P/S-bound active enhancers and promoters of differentially affected zygotic genes. Heatmaps are centered and ranked as in (A). inhibitor: Pol II inhibitors (triptolide + flavopiridol). (D) Representative genome tracks of Pol II, p300, Brd4, and NPS binding, Omni-ATAC and histone acetylation across core histones in WT and MZnps embryos. See also Figure S2.