Table 2.

Summary of single-cell isolation protocols for cartilage in reported studies using scRNA-seq technique

Reference	Tissue	Species	Treatment	Number of profiling cells
Quanbo Ji, et al. [34]	Cartilage from 10 knee OA patients	Human	0.25% Trypsin–EDTA for 30 min and then 0.2% type II collagenase (Sigma-Aldrich) for 4 h	1600
Fiorella Carla Grandi, et al. [35]	OA samples from patients undergoing total joint replacement	Human	Collagenase II and IV (2.5 mg/ml each; Worthington Biochem) overnight	Not reported
Junxiang Li, et al. [36]	The distal cartilage structure at postnatal day 7	Mouse	0.2% collagenase II for 2 h	217
Vikram Sunkara, et al. [37]	Femur of 12 weeks old mice	Mouse	10 mg/ml Collagenase II (Nordmark) supplemented for 4 h	7133
Koji Mizuhashi, et al. [38]	Femur growth plate cells at postnatal day 2	Mouse	Liberase TM (Sigma/Roche) for unclear time	18,000
Natalie H Kelly, et al. [39]	Hindlimb bud of embryonic mice	Mouse	Collagenase Type II (Worthington-biochem, Lakewood, NJ) and pronase (EMD Millipore, Billerica, MA) in 15 min increments with agitation for up to 1 h	9466