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. Author manuscript; available in PMC: 2022 Dec 24.
Published in final edited form as: Sci Immunol. 2022 Jun 17;7(72):eabe0584. doi: 10.1126/sciimmunol.abe0584

Figure 4.

Figure 4.

Stimulation of the cold-sensing receptor TRPM8 activates skin ILC2s. (A) Percentages and numbers of Lin− ILCs in the menthol versus control (ethanol)-treated skin of WT mice. (B-E) Comparison of the percentage and number of CCR10+MHCII+ ILCs (B), CCR10+Clec12a+ ILCs (C), CCR10−CD81+ (D) and CCR10−ST2+ (E) ILCs in the menthol versus control (ethanol)-treated skin of WT mice. (F) Analysis of IL-5 expression by ILCs of the menthol versus control (ethanol)-treated skin of WT mice. (G) Analysis of CCR10 and CD81 expression on skin ILCs in WT mice versus TRPM8−/− mice treated with menthol daily for 2 weeks. (H) Analysis of CCR10 and ST2 expression on ILCs of the menthol-treated skin of WT versus TRPM8−/− mice. Both WT and TRPM8−/− mice carry a CCR10-knockout/EGFP-knockin (CCR10+/EGFP) allele for purpose of identifying CCR10+ cells with EGFP. One dot is one sample. *P<0.05, **P<0.01. ***P<0.001, ***P<0.0001, NS: no significant difference. Statistical significance was determined by unpaired T test for the panel F, paired T test for panels A-E, G-H.