FIG 8.
The divergent responses of IFN and ISGs induced by PRCoV infection in 3D AOs. (A) Heatmap showing the expression of IFN and IFN receptors in 3D AOs infected with or without PRCoV. (B to D, F to I). Verification of the expression of the listed differentially expressed genes between PRCoV-infected AOs by RT-qPCR: IFN-β (B), IFN-λ (C), IFNAR1 (D), IL10RB (F), IFNLR (G), ISG15 (H), and OASL (I). (E) Heatmap showing the expression of randomly selected upregulated ISGs in PRCoV-infected 3D AOs. (J) PRCoV is sensitive to exogenous IFN-β. All the data were calculated using the 2-ΔΔCT method, normalized to GAPDH expression, and compared with mock AOs. Error bars denote the deviations indicated by *: *, P < 0.05; **, P < 0.01; ***, P < 0.001.