FIG 3.

Astrovirus Yuc8 infection stimulates secretion of extracellular vesicles in Caco-2 cells. Fractions were purified from supernatants of Caco-2 cells infected with astrovirus Yuc8 (MOI of 5) or mock infected, 18 h postinfection and processed by differential centrifugation, after pelleting at 20,000 g (LEV) (A), and obtained after PEG 6000 precipitation (SEV) (B). Both fractions were resuspended in PBS and used for nanoparticle tracking analysis in the NanoSight NS300. In each experiment five videos were recorded and used for analysis. Distribution of particle-vesicle size (hydrodynamic diameter in nm) and concentration (particles/mL) from 3 to 5 independent experiments are shown. Vesicles purified from mock infected cells are represented by blue line, vesicles from Yuc8 infected cells are represented by red line. (C) Comparison of the mean number of particles present in LEV and SEV fractions shown in A and B. All results are expressed as the mean of the whole concentration of particles ± standard error of the mean of three independent experiments. Statistical analysis was done using two-way ANOVA *, P < 0.05.