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Bovine enteroids infected with NSP3 mutant viruses. Three-dimensional bovine enteroids were infected with the panel of NSP3 mutants, or mock at an approximate multiplicities of infection of 10 PFU per cell and fixed at 24 hpi, or at 0 hpi for WT as a control for background signal. Cells were stained for RV VP6 (A) and RBD of SARS-CoV-2 spike (B). Scale bar represents 10 μm.
