TABLE 3.
SARS-CoV-2 spike amino acid sequences selected for insertion (61, 98, 99) into the hypervariable region of SA11 VP4 and tagging of RF NSP3a
| SARS-CoV-2 peptide insertions | Amino acid sequence (N to C terminal) |
|---|---|
| SA11 VP4 construct | |
| HR2 | EIDRLNEVAKNLNESLIDLQELGKYEQY |
| NTD | KSFEIDKGIYQTSNFRVV |
| RBM.1 | SNNLDSKVGGNYNYLYRL |
| RBM.2 | FRKSNLKPFERDISTEIYQAGS |
| RF NSP3 construct | |
| RBM | NSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPY |
| T2A_RBM | EGRGSLLTCGDVEENPGPNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPY |
| RBD | LCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGP |
| T2A_RBD | EGRGSLLTCGDVEENPGPLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGP |
a
Sequence of the T2A peptide (66) is underlined.