TABLE 4.
Primary and secondary antibodies used in this studya
| Antibody | Catalog no. (supplier) | Clonality/host species or conjugate | Dilution | Used in |
|---|---|---|---|---|
| Primary antibody | ||||
| RV VP6 | Ab181695 (Abcam) | Monoclonal/mouse | 1 in 1,000 | Western blotting Immunofluorescence |
| Alpha-tubulin | NB600-506 (Novus Biologicals) | Monoclonal/rat | 1 in 5,000 | Western blotting |
| SARS-CoV-2 Spike Protein | DA123 (MRC protein phosphorylation and Ubiquitylation unit, Dundee) | Polyclonal/sheep | 1 in 100 | |
| SARS-CoV-2 Spike RBD | PA5-114451 (Invitrogen) | Polyclonal/rabbit | 1 in 100 | ELISA |
| 1 in 1,000 | Immunofluorescence | |||
| 1 in 1,000 | Western blotting | |||
| Secondary antibody | ||||
| Donkey anti-mouse | A32766 (Invitrogen) | Alexa Fluor 488 | 1 in 1,000 | Immunofluorescence |
| Donkey anti-mouse | 926-32212 (LI-COR) | Alexa Fluor 800 | 1 in 10,000 | Western blotting |
| Goat anti-rat | 926-68076 (LI-COR) | Alexa Fluor 680 | 1 in 10,000 | |
| Rabbit anti-sheep | SA5-10060 (Invitrogen) | Alexa Fluor 800 | 1 in 10,000 |
a
RV, rotovirus.