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. 2022 Jul 6;96(14):e00545-22. doi: 10.1128/jvi.00545-22

FIG 1.

FIG 1

Evaluation of the H108R protein across ASFV isolates. (A) Amino acid alignment representing the diversity of the H108R protein of ASFV in the field. Residues in white spots represent changes between amino acids with different charges. Conservation plot scores reflect the nature of the change in specific sites, and high scores are associated with changes with similar biological properties. Alignment was produced using the Jalview version 2.11.1.4 software package. (B) Phylogenetic analysis conducted via the maximum likelihood method and the Tamura-3 parameter model, showing the diversity of the H108R gene of ASFV in the field. Based on the cluster distribution, isolates were categorized into three main groups. Numbers above internal branches represent bootstrap values (1,000 repetitions). (C) The graphic represents the dN (rate of evolution at nonsynonymous sites) to dS (rate of evolution at synonymous sites) ratio (dN-dS) at specific codon sites in the H108R gene of ASFV. Blue and red asterisks represent codon sites evolving under diversifying and purifying selection, respectively. Analyses were conducted using the evolutionary algorithms FEL and MEME, using cutoff values of P = 0.1.