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(A) Restriction endonuclease maps of a plasmid clone harboring the gene for chitinase (Chi92). (B) Domain structures of Chi92 and various derivative recombinant proteins used in this study. The numbers refer to the positions of amino acids. Abbreviations: SP, signal peptide; Chi92-N, all-β-strand N-terminal region; GHF 18, catalytic domains of glycosyl hydrolase family 18; A, unknown-function region. ChBD, chitin-binding domain; GST, glutathione S-transferase.
