FIG. 4.

SDS-PAGE analysis of truncated Chi92 derivatives and GST fusion proteins. (A) Chi92ΔCII, Chi92ΔCICII, and Chi92ΔACICII purified by Q Sepharose and Sephacryl S-200 chromatography as described in Materials and Methods. Lanes: 1, Chi92 purified from E. coli JM109(pHX); 2, Chi92ΔCII purified from E. coli JM109(pChi87); 3, Chi92ΔCICII purified from E. coli JM109(pChi81); 4, Chi92ΔACICII purified from E. coli JM109(pChi60). (B) GST-CICII and GST-CI purified by glutathione Sepharose 4B chromatography. Lanes: 1, crude cell extract from E. coli XL1-Blue(pGEX-CI); 2, purified GST-CI; 3, crude cell extract from E. coli XL1-Blue(pGEX-CICII); 4, purified GST-CICII. The proteins were analyzed by SDS-PAGE, and the gel was stained with Coomassie brilliant blue R-250. Molecular mass standards were run in lanes M.