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. 2022 Jul 5;96(14):e00608-22. doi: 10.1128/jvi.00608-22

TABLE 2.

Primers used for RT-qPCR analysis

Target gene Primer sequence (5′–3′)
Forward Reverse
ACE2 human GGACCCAGGAAATGTTCAGA GGCTGCAGAAAGTGACATGA
ACE2 FLG TGGGACTCTACCGTTCACTTA GCTTCATCTCCCACCACTTT
ACE2 Mm TGCTTATGTCAGGGCAAAGT CCCACATATCACCAAGCAAATG
ACE2 Nn CAGTCCTGGGATGCAGATAAG TGGCTCAGTTAGCATGGATTTA
GAPDH human GGTCGGAGTCAACGGATTTG ACTCCACGACGTACTCAGCG
GAPDH FLG TCATCAACGGAAAGTCCATCTC ACATACTCAGCACCAGCATC
GAPDH Mm GTAGTGAAGCAGGCATCAGAG GGAGTGGGTGTCACTGTTAAA
GAPDH Nn CCTGTTCGTCAGACAGCCTT TTGATGGCGACAACTTGCAC
IFIH1 human ACA CGT TCT TTG CGA TTT CC ACC AAA TAC AGG AGC CAT GC
IFIH1 Mm/FLG GGAGTCAAAGCCCACCATCT TCCAGACCTTCTTCTGCCAC
IFIH1 Nn TTTGCCAAGTGAGCCCAATG AAGCGGTCTTTGCGATTTCC
OAS human GAGCTCCTGACGGTCTATGC TTCGTGAGCTGCCTTCTCAG
OAS pan-bat GGAAGGAGGGCGAGTTCTC GGTACCAGTGCTTGACCAGG
SARS-CoV-2 nsp12 polymerase GGTAACTGGTATGATTTC CTGGTCAAGGTTAATATAGG