FIG 1.

Mouse model of T cell-specific IL-17RA deficiency. (A) Representative PCR products of genotyping reactions designed to detect the presence of the LoxP sites for the conditional IL-17RA allele and presence of the dLck Cre allele. For the floxed IL-17RA allele, the wild type (WT) is at ~304 bp and the mutant is at ~350 bp. The internal positive control is at 200 bp in the dLck Cre reaction, while the transgene is at 300 bp. C57BL/6 (BL6) mice were genotyped as a negative control (B). Cellular expression of IL-17RA in CD3⁺ T cells and CD3⁻ cells from the spleen was determined by flow cytometry. (C and D) Absolute numbers of CD3⁺ T cells in the spleen (C) and peritoneal cavity (D) isolated from naive dLck Cre-positive and dLck Cre-negative littermates. Data were pooled from 4 to 6 independent experiments, with each symbol representing an individual mouse.