FIG 3.

Moderate HIV-1 reactivation following LRAs treatment of latently infected Hpt1-b renal cells. Latently infected renal cells were flow sorted 2 weeks postinfection and cultured for an additional week before LRAs treatment. Cells were then treated for 24 h with the indicated LRAs or DMSO either once (a) or twice (b). FACS analyses of representative single and double treatments are shown. Histograms represent the fold change in GFP expression compared to untreated controls for the single (c) or double treatments (d). Bars represent mean+SD of the percentage of GFP+ population in three independent experiments. Asterisks indicate a significant difference (P value <0.05; nonparametric Kruskall-Wallis test) between LRA treated cells and DMSO.