FIG 5.

HIV-1 reactivation following JQ1 treatment of latently infected primary HRCEp cells. Latently infected primary HRCEp cells were flow sorted 11 days postinfection and cultured for an additional 8 days before treatment with JQ1. (a) Gating strategy for flow cytometry sort of latently infected HRCEp cells (day 11 postinfection). (b) Latently infected (mCherry only) flow-sorted HRCEp cells were reseeded and the expression of mCherry and GFP was analyzed at 8 days post-sort. Cells were then treated twice with JQ1 for 24 h and imaged 24 h after the first treatment, and 24 and 48 h after the second treatment. (b) Shown are images of mCherry and GFP positive HRCEp cells from eight separate fields taken at the indicated time points from untreated and JQ1 treated cells. Number of GFP+ cells present in each field are indicated in the top left corner of each image. (c) Fold change in number of GFP+ cells in untreated and JQ1 treated cells at the indicated time points.