FIG 1.

HCMV infection causes transcriptomic changes to trafficking proteins. (A) RNA was isolated from HCMV-infected primary human monocytes at 4 hpi from 6 donors and converted to cDNA (GEO accession number GSE11408). A microarray was then performed on whole cell transcripts, which were analyzed by GEO2R software and compared to mock infected cells. A volcano plot was constructed of transcripts upregulated (red) and downregulated (blue) relative to mock. P value versus fold change relative to mock is plotted. (B) RNA was isolated from mock and HCMV-infected primary human monocytes (3 donors) at 4 hpi, 24 hpi, 2, 4, and 6 wpi and prepared for RNAseq (GSE206198). Paired end sequencing was performed (>25 × 10⁶ reads) and aligned to HCMV Lisa and human genome to determine differential expression (FDR=0.05). Transcripts per million (TPM) were log 2 normalized for gene level analysis. Stx6 gene expression in mock (blue) and HCMV-infected monocytes (red) are shown.