FIG 2.

HCMV infection increases Stx6 protein levels. Primary human monocytes (A and B), HEL fibroblasts (C and D), and ARPE-19 epithelial cells (E and F) were synchronously infected with HCMV TB40/E UL32-GFP (MOI = 5). Protein was harvested 30 mpi, 1 hpi, and/or 2 hpi, 4 hpi, 24 hpi depending on the experiment. Protein lysates were immunoblotted for Stx6 and actin. Protein band densities were quantified by LI-COR Image Studio software, normalized to mock, and expressed as fold change over mock. Single factor ANOVA was performed for statistical significance. *, P < 0.05; **, P < 0.01; ***, P < 0.001. N = 3 per cell-type.