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Comprehensive analyses of EBV protein interactions. (A) Schematic illustration of NanoBiT screening. All 69 EBV genes were cloned into LargeBiT and SmallBiT vectors. HEK293T cells were transfected with the NanoBiT plasmids in different pair combinations, as indicated. Luciferase activity was measured 24 h after transfection. (B) Heatmap showing the relative luminescence, which was calculated by dividing the luminescence of each combination by that of the negative control.
