Extended Data Figure 5. Specificity of viral injections and cell type identity.

(a) Example of viral injection specificity that qualified for inclusion in the study. Note viral expression in the VMPO and absence in surrounding brain areas. Injections found to be more broadly dispersed were not included in analysis. (b-d) Chemogenetic activation of Calcr+ and Gal+ neurons in the VMPO. (b) CNO injection elicited strong increase in body temperature in both genetic backgrounds (n=6 mice/group). (c) Activation of Gal-Cre but not Calcr-Cre increased preferred temperature (n=7mice/group). (d) Activation of Calcr-Cre (n=6 mice) but not Gal-Cre (n=8) decreased chow consumed (saline n=6 mice). (e) DTA-mediated Ablation of VMPO^LPS neurons had no effect on circadian temperatures, dark bar indicates dark phase, WT mice were used as controls. (f-g) Effect of DTA-mediated ablation of VMPO neurons in Saline-TRAP mice had no effect on body temperature or preferred temperature following LPS injection (n=7 mice/group). (h) Overlap of warm-TRAP reporter expression with markers of warm-sensitive neurons adcyap1 and sncg. (i) Overlap of hunger-TRAP-mediated reporter expression (magenta) with markers of appetite-increasing neurons, agrp, or appetite-decreasing neurons, pomc, (green). Scale bars = 200μm. All error bars = SEM. For all graphs * =p<0.05, ** =p<0.01, *** =p<0.001