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. 2022 Jul 27;13:4350. doi: 10.1038/s41467-022-31929-6

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Calu-3 cells were infected with 200–400 focus forming units (FFU) of each virus and incubated for 2 days in media with or without sera. The viruses were collected from Calu-3 supernatant at 2 days post inoculation and titrated by FFU assay. The geometric mean viral titers of variants under different treatment conditions are represented in the graph as bars with geometric standard deviation. Bar graphs present the titers of each variant under different treatment conditions. Error bars representing titer differences are marked as *, representing p < 0.05, compared to the no sera control within each virus group. Two-tailed Wilcoxon matched-pairs signed-rank test was used for statistical significance analysis and the statistics and P value are summarized in Supplementary Table 2. Source data are provided as a Source Data file. Titer fold changes (reductions) compared to the no sera control are shown on the top of the panel. a The sera for incubation were pooled from the individual sera used in Fig. 2a and diluted to 2X or 5X concentration (diluted sera titer FRNT₅₀ = 2 or 5 against 614D reference virus). N = 6 or 12 viral titers over 2 biologically independent sera pools in 1–2 independent experiments. b The sera for incubation were pooled from the individual sera used in Fig. 2a (post-second dose) and diluted to 10X or 20X concentration (diluted sera titer FRNT₅₀ = 10 or 20 against 614D reference virus). N = 18 viral titers over 2 biologically independent sera pools in 1–2 independent experiments. The fold change for the 614D-20X sera group is not determined (ND) as the average viral titer of that group is below the LOQ. c The sera for incubation were pooled from the individual sera used in Fig. 3b (post-third dose) and diluted to 10X or 20X concentration (diluted sera titer FRNT₅₀ = 10 or 20 against 614D reference virus). N = 18 viral titers over 2 biologically independent sera pools in 1–2 independent experiments. The fold change for the 614D-20X sera group is not determined (ND) as the average viral titer of that group is below the LOQ. Yellow bars, viral titers in the absence of sera; blue bars, viral titers in 2X or 10X sera; purple bars, viral titers in 5X or 20X sera. Limit of quantification LOQ = 240 FFU/mL.