FIGURE 3.

Effect of EWDE on dermal papilla inductivity in hair follicles remaining in anagen after the culture. (a) Versican expression was measured in dermal papilla of vehicle‐ and EWDE‐treated HFs. Mean ± SEM, n = 5–10 anagen HFs from three donors (independent experiments); Mann–Whitney test, n.s. (b) Versican expression was measured in dermal papilla of vehicle‐ and EWDE‐treated HFs from female donors. Mean ± SEM, n = 6–7 HFs from two female donors (independent experiments); Mann–Whitney test, *p = 0.0424. (c) Alkaline phosphatase activity was measured in dermal papilla of vehicle‐ and EWDE‐treated anagen HFs. Mean ± SEM, n = 6–9 HFs from three donors (independent experiments); Mann–Whitney test, n.s. (d) Alkaline phosphatase activity was measured in dermal papilla of vehicle‐ and EWDE‐treated HFs from female donors. Mean ± SEM, n = 3–6 anagen HFs from two female donors (independent experiments); Mann–Whitney test, *p = 0.0238. (e) FGF‐7 expression was measured in dermal papilla of vehicle‐ and EWDE‐treated HFs. Mean ± SEM, n = 6–10 HFs from three donors (independent experiments); Mann–Whitney test, n.s. (f) FGF‐7 expression was measured in dermal papilla of vehicle‐ and EWDE‐treated HFs from female donors. Mean ± SEM, n = 4–7 HFs from two female donors (independent experiments); Mann–Whitney test, n.s. (g) Representative pictures of versican, alkaline phosphatase and FGF‐7 immunostaining in vehicle‐ and EWDE‐treated HFs. Immunoreactivity was quantified in dermal papilla (demarcated areas). Blue and green dots represent hair follicles from female donors, and red dots represent hair follicles from the male donor. HFs, hair follicles; DP, dermal papilla; and n.s., not significant. Scale bar: 100 μm [Colour figure can be viewed at wileyonlinelibrary.com]