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PM1 standard curve. Real-time analysis of serial 10-fold dilutions of PM1 DNA (A) or PM1 cells (B). The CT values are plotted against the corresponding PM1 cell numbers in the PCR. Template DNA was extracted from 1.8 × 107 CFU of PM1 corresponding to 3 × 106 CFU/PCR and was diluted 10-fold (A). For the second curve (B) DNA was extracted from serial dilutions of PM1 cells mixed with E. coli cells until 108 total CFU/ml was reached.
