TABLE 1.
Bacterial strains used to test the specificity of the PM1 TaqMan assay
| Bacterial straina | Medium | CT valueb |
|---|---|---|
| PM1 | MSM | 23.2 |
| E. coli K-12 ATCC 10798 | ATCC medium 3 | 40 |
| Bacillus subtilis ATCC 6051 | ATCC medium 3 | 40 |
| P. putida ATCC 12633 | ATCC medium 3 | 38.1 |
| S. lactis ATCC 19435 | ATCC medium 17 | 38.68 |
| Staphylococcus aureus ATCC | ATCC medium 3 | 40 |
| A. facilis ATCC 11228 | ATCC medium 81 | 37.03 |
| H. flava ATCC 33667 | ATCC medium 3 | 40 |
| Micrococcus luteus ATCC 4698 | ATCC medium 18 | 40 |
| Acinetobacter calcoaceticus | ATCC medium 3 | 40 |
| Azomonas agilis ATCC 7494 | ATCC medium 11 | 40 |
| A. commune DSMZ 11901 | DSMZ medium 464 | 39.55 |
| Mycobacterium sp. | ATCC medium 90 | 40 |
| Gr(+) bacterial isolate | 0.1 × TS | 40 |
| Gr(−) bacterial isolate | 0.1 × TS | 40 |
| Nitrosomonas europaea ATCC 19718 | ATCC medium 221 | 40 |
a
Strains were obtained from American Type Culture Collection (ATCC; Manassas, Va.) or Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH (DSMZ; Braunschweig, Germany).
b
CT values obtained when 1,000 pg of DNA was used as a DNA template in the TaqMan PCR.