TABLE 3.
TaqMan PM1 detection in spiked sediment or groundwater microcosms
| Samplea | Mean CTb | Standard deviation | CV for CT (%) | CFUc |
|---|---|---|---|---|
| Sediment | 39.7 | 0.70 | 1.8 | ND |
| Sediment plus MSM | 39.7 | 0.64 | 1.6 | ND |
| Sediment plus 106 PM1 | 19.8 | 0.14 | 0.7 | 8.1 × 106 ± 0.6 × 106 |
| Sediment plus 107 PM1 | 17.8 | 0.32 | 1.8 | 2.0 × 107 ± 0.4 × 107 |
| Control groundwater | 34.3 | 0.61 | 1.8 | <DL |
| Groundwater plus 106 PM1 | 19.7 | 0.32 | 1.6 | 1.1 × 106 ± 0.4 × 106 |
| Groundwater plus 108 PM1 | 15.2 | 0.35 | 2.3 | 1.1 × 108 ± 0.5 × 108 |
a
Sediment or groundwater microcosms with different numbers of PM1 cells added.
b
Mean CT represents replicate samples from each of three microcosm bottles times two PCR replicates (n = 12).
c
CFU of PM1 per gram of sediment or per milliliter of groundwater; CFU were calculated by using the standard curve (Fig. 1B). ND, not detectable. <DL, below the detection limit of 180 CFU/ml.