TABLE 4.
PM1 detection in groundwater DNA samples collected from an MTBE-contaminated plume at Port Hueneme, California
| Samplea | Mean CTb | Standard deviation | CV for CT (%) | CFU/ml of groundwaterc |
|---|---|---|---|---|
| BIBD | 23.5 | 0.12 | 0.5 | 2.0 × 104 ± 0.3 × 104 |
| B1-D | 27.6 | 0.27 | 1.0 | 1.4 × 103 ± 0.4 × 103 |
| B2-D | 24.5 | 0.08 | 0.3 | 1.4 × 104 ± 0.3 × 104 |
| B1-S | 23.1 | 0.09 | 0.4 | 2.5 × 104 ± 0.2 × 104 |
| B4-S | 23.5 | 0.25 | 1.1 | 1.9 × 104 ± 0.4 × 104 |
| B4-D | 24.6 | 0.13 | 0.5 | 9.4 × 103 ± 0.4 × 103 |
| B5-S | 28.0 | 0.25 | 0.7 | 9.9 × 103 ± 0.3 × 103 |
| CBC61 | 35.5 | 0.33 | 0.9 | <DL |
| CBC10 | 39.0 | 0.60 | 1.6 | <DL |
| PHB4 | 32.9 | 0.39 | 1.2 | <DL |
a
B1-D, B2-D, and B4-D correspond to deep wells and B1-S, B4-S, and B5-S, correspond to shallow wells in the field test plot where strain PM1 was injected. BIBD, injection bed. CBC10 is (649 m) upstream and PHB4 is (623 m) downstream of the test plot. CBC61 is close (40 m) to the test plot.
b
Mean CT represents two PCR replicates of two replicate groundwater samples (n = 4).
c
CFU were calculated by using a standard curve, shown in Fig. 1B. <DL, below the detection limit of 180 CFU/ml.