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. 2021 Feb 12;178(7):1507–1523. doi: 10.1111/bph.15377

FIGURE 2.

FIGURE 2

ABHD6 assay based on 4‐MUH hydrolysis in HEK‐293T cells overexpressing ABHD6. (a) ABHD6 hydrolyses 4‐MUH producing 4‐MU, a highly florescent compound (excitation 355 nm; emission 460 nm). (b) 4‐MUH hydrolysis by cells expressing empty vector (EV) or ABHD6 (n = 7). (c) ABHD6 activity dependent on 4‐MUH concentration at t = 10 min (n = 6). (d) ABHD6 activity inhibition by WWL70 at t = 10 min (n = 8). (e) 2‐AG competition with 4‐MUH in cells expressing ABHD6 (n = 6). Data shown are means ± SD. *P < 0.05, significantly different from EV; two‐way ANOVA followed by Bonferroni's post hoc correction