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. 2022 Jul 27;8(30):eabo4577. doi: 10.1126/sciadv.abo4577

Fig. 2. SMAD4 regulates CD8+ T cell activation and effector function.

Fig. 2.

(A) Heatmap of selected differentially expressed genes showing OVA-specific CD8+ TILs from WT mice versus that from Cd8CreSmad4fl/fl mice (WT versus KO). (B) Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of SMAD4 up-regulated (SMAD4 abrogation down-regulated) genes between OVA-specific CD8+ TILs from WT and Cd8CreSmad4fl/fl mice. TH, T helper cell. (C) Gene set enrichment analysis of effector genes in chronic LCMV infection (12). Genes from the left to right of the rank-ordered list are enriched in WT and Smad4-deficient (KO) groups, respectively. (D) Gene set enrichment of genes describing for exhausted CD8+ T cells in mouse autochthonous melanoma, relative to naive CD8+ T cells (13). (E) Gene set enrichment of genes describing for terminally exhausted CD8+ T cells in B16-OVA tumor model, relative to progenitor exhausted CD8+ T cells (14). NES, normalized enrichment score.